Figure 4
From: Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44
The transcriptional analysis of acid-tolerant genes hdeA, hdeB murG and ldh in wild type F44 and the engineered strain F44 (ABGL) by qRT-PCR.
The total RNA extracted from cells which were incubated at pH 7.0, 6.0, 5.0 and 4.0 in seed medium, respectively. The obtained cDNA was subjected to qRT-PCR. The 16S rRNA gene was used as the internal control gene and hdeA, hdeB murG and ldh in wild type F44 were set as the guide sample.
