Figure 7
From: Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44
Fed-batch culture analysis of cell growth (a), pH of broth (b) and nisin production (c) with F44, transformants with single expression of murG and combinatorial expression of hdeA, hdeB murG and ldh genes, respectively. The culture temperature was maintained at 30 °C. Concentrated sucrose (500 g/L) was fed at a constant rate of 2 mL/h between 6–12 h during fermentation process. Samples were taken at every 2 h.
