Table 1 Summary of the information about acid tolerance genes and lactic acid synthetic genes. L. lactis F44 was actually obtained by genome shuffling of L. lactis YF11 (CGMCC NO. 7.52).
From: Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44
Gene ID | Gene | Organism | Function |
|---|---|---|---|
Obstacle of extracellular H + | |||
– | murA | Lactococcus lactis F44 | UDP-N-acetylglucosamine 1-carboxyvinyltransferase |
– | murG | Lactococcus lactis F44 | UDP-N-acetylglucosamine–N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase |
gi|944811 | cfa | Escherichia coli DH5α | Cyclopropane-fatty-acyl-phospholipid synthase |
Consumption of intracellular H + | |||
– | mleS | Lactococcus lactis F44 | Malolactic enzyme |
– | mleP | Lactococcus lactis F44 | Malate/lactate antiporter |
– | gadB | Lactococcus lactis F44 | Glutamate decarboxylase |
– | gadC | Lactococcus lactis F44 | Glutamate/gamma-aminobutyrate antiporter |
– | gadBC | Lactococcus lactis F44 | Glutamate decarboxylation system |
gi|948643 | cadA | Escherichia coli DH5α | Lysine decarboxylase, inducible |
gi|948654 | cadB | Escherichia coli DH5α | Probable cadaverine/lysine antiporter |
Exportation of intracellular H + | |||
– | atpD | Lactococcus lactis F44 | ATP synthase subunit beta |
gi|948244 | atpD | Escherichia coli DH5α | ATP synthase subunit beta |
– | atpG | Lactococcus lactis F44 | ATP synthase gamma chain |
gi|948243 | atpG | Escherichia coli DH5α | ATP synthase gamma chain |
Proteins refolding | |||
gi|948025 | hdeA | Escherichia coli DH5α | Acid stress chaperone HdeA |
gi|948026 | hdeB | Escherichia coli DH5α | Acid stress chaperone HdeB |
– | hdeAB | Escherichia coli DH5α | Periplasmic acid stress protein |
Alternation of carbohydrate metabolic pathway | |||
– | pfk | Lactococcus lactis F44 | 6-phosphofructokinase |
gi|9459266 | pfk | Lactobacillus casei Zhang | 6-phosphofructokinase |
– | pyk | Lactococcus lactis F44 | pyruvate kinase |
gi|9459267 | pyk | Lactobacillus casei Zhang | pyruvate kinase |
– | ldh | Lactococcus lactis F44 | L-lactate dehydrogenase |
gi|9460421 | ldh | Lactobacillus casei Zhang | L-lactate dehydrogenase |
