Figure 3
Mid represses the expression of wg.
(a–c) The expression of Mid (a) and Wg (b) in the wild-type wing disc. (c) Merged image of (a,b). (d–f) Magnified images of the partial region in (a–c) are shown. (g–i) Wg expression is activated in a mid mutant clone (mutant clone lack GFP signal and marked by a broken line) induced in the DP cells of the wing disc. The wing disc from a third instar larva of hs-FLP; FRT42D, Ubi-GFP/FRT42D, mid1 is stained with anti-GFP antibody (g), anti-Wg antibody (h). (i) Merged image of (g,h). (j,k) Activation of the Wg expression in the wing disc of sd-Gal4; UAS-mid-RNAi (k) compared with that of yw (j). (l) qPCR analyses of the wg mRNA level between yw and sd > mid-RNAi. Error bar, SEM from three independent experiments. Student’s tests, **p < 0.01. (m–o) Somatic clone overexpressing Mid display downregulation of Wg (clone with GFP signal is marked by broken line). The wing disc from a third instar larva of hs-FLP; UAS-mid/act > y + > gal4; UAS-GFP is stained with anti-GFP antibody (m), anti-Wg antibody (n). (o) Merged image of (m,n).
