Figure 4

Mid represses the expression of arm.
(a) The wing disc of wild type is stained with the anti-Arm antibody. (b,c) qPCR analyses of wg and arm mRNA level in yw and sd > mid. Error bar, SEM from three independent experiments. Student’s tests, ***p < 0.001. (d–f) The expression of Arm is repressed (marked by brackets) in the wing disc of sd-Gal4; UAS-mid. (g–i) Arm expression is activated in a mid mutant clone (mutant clone lack GFP signal and is marked by broken line) induced in the DP cells of the wing disc. The wing disc from a third instar larva of hs-FLP; FRT42D, Ubi-GFP/FRT42D, mid1 is stained with anti-GFP antibody (g), anti-Arm antibody (h). (i) Merged image of (g,h). (j–l) Somatic clone overexpressing Mid display downregulation of Arm (clone is marked with GFP signal and is marked by broken line). The wing disc is stained with anti-GFP antibody (j), anti-Arm antibody (k). (l) Merged image of (j,k).