Figure 6

Mid represses the expression of hh.
(a–c) Genetic interaction between mid and hh. Adult wings of mid1/+; +/+ (a) /+; hhmrt/+ (b) and mid1/+; hhmrt/+ (c). Note ectopic vein L3 (L3*) in (b) and ectopic veins L2 (L2*) and L3 (L3*) with enlarged anterior compartment in (c). Scale bars: 200 μm. (d) Percentage of the phenotype in (a–c). (e,f) The wing discs of wild type and sd > mid are stained with the anti-Hh antibody. The expression of Hh is repressed upon overexpression of mid (marked by broken lines in (f)). (g–i) The wing disc of sd-Gal4;UAS-mid;hh-lacZ is stained with the β-gal antibody. The expression of hh-lacZ reporter is repressed (marked by broken lines). (j–l) Somatic clone overexpressing Mid display downregulation of Hh (marked by broken lines). The wing disc from a third instar larva of hs-FLP; UAS-mid;hh-lacZ/act > y + > gal4; UAS-GFP is stained with anti-GFP antibody (j), β-gal antibody (k). (l) Merged image of (j,k). (m,n) qPCR analyses of the hh and dpp mRNA levels in yw and sd > mid.