Figure 6

NadA binds CHO cells expressing hLOX-1.

FACS plots representative of LOX-1 (YFP) expression in CHO cells and cells able to bind NadA (APC). The gating strategy was designed to separate the cells of interest from large aggregates and debris [initial gate on forward scatter (FSC) versus side scatter (SSC) plot], deplete dead cells (Live/Dead Aqua staining) and doublets/aggregates (standard gates on both FSC-width and SSC-width) (data not shown). Simple gating by quadrants allowed defining the absolute percentages of cells positive for LOX-1 only (32%, bottom left) and double positive cells (30%, bottom right) representing the cells able to bind recombinant NadA. The upper right panel shows that not-transfected cells are not able to bind NadA. The upper left panel shows the non-transfected cells.