Figure 4 | Scientific Reports

Figure 4

From: Mycobacterium tuberculosis Rv1152 is a Novel GntR Family Transcriptional Regulator Involved in Intrinsic Vancomycin Resistance and is a Potential Vancomycin Adjuvant Target

Figure 4

Construction of the â–³MSMEG_5174 and â–³MSMEG_5174::Rv1152 strains.

(A) confirmation of MSMEG_5174 gene knockout by PCR amplification from WT and â–³MSMEG_5174 strains. M, Marker DL10000; 1, PCR band from â–³MSMEG_5174 strain; 2, PCR band from WT strain. (B) A map of the sequencing of PCR amplification products and DNAMAN alignment showing 28 bp DIF in â–³MSMEG_5174 strain. (C) Western blot showed that the Rv1152 gene was expressed in â–³MSMEG_5174 strain.

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