Table 2 Primers used in the study.
Primer | Description | Sequence of primers (5′-3′) |
|---|---|---|
Rv1152-F1 | Construct recombinant MS_Rv1152 strain | GGATCCCGCCAGGCGCGCTCAGCCG |
Rv1152-R1 | AAGCTTCCGAAGGCGACCGTGGTGG | |
KO-F1 | Amplify MSMEG_5174 upstream sequence | TATGGATCCTACGTGGAGCTGCG |
KO-R1 | GTTGCATCGATTCAGTCGTCCGG | |
KO-F2 | Amplify MSMEG_5174 downstream sequence | AAGCTTCGCTCGGGGTTGACCTCGA |
KO-R2 | TCGGCTGAGCGCGCCTGGCGGGATCCTTCGGTCACACCCTTGAGTATG | |
Rv1152-F2 | Confirm dif sequence in mutation | AACGTGGGCACGCCGCTTT |
Rv1152-R2 | TGTCATATGTCAGTCCAGCGCGG | |
Lat-F1 | Construct recombinant MS_Lat strain | TGCTTCATCGCCGAACCCATCCA |
Lat-R1 | CGAACGAGACCACATCGGGCATCACA | |
Rv0251c-F1 | Construct recombinant MS_Rv0251c strain | GACGGATCCATGAACAATCTCGCA |
Rv0251c-R1 | CGCGCCATATGCTACTTCGTGAT | |
Rv1285-F1 | Construct recombinant MS_1285 strain | CTGGTCGGATCCATGGCAATAAC |
Rv1285-R1 | CTGGTCGGATCCATGGCAATAAC | |
Rv0563-F1 | Construct recombinant MS_0563 strain | GGAGGACACGGATCCATGACTTGGC |
Rv0563-R1 | GGAATTCCATATGTCAGCCGCGCGCCAT | |
MSMEG_0424-F1 | Construct recombinant MSMEG_0424 overexpressing strain | AGGCGGATCCATGAGCACGCTGATGAA |
MSMEG_0424-R1 | GCGGGTTTCATATGTTACCGGCTCTCG | |
MSMEG_4979-F1 | Construct MSMEG_4979 overexpressing strain | AACGGATCCATGACCGCAGTCGACAG |
MSMEG_4979-R1 | GCGTCGCCATATGTCAGAAATACCCC | |
MSMEG_1134-F1 | Construct MSMEG_1134 overexpressing strain | GACGGATCCATGACGTGGAATCC |
MSMEG_1134-R1 | CGCCATATGTCAGTAGTACGAGTCC |
