Figure 1

Spermatogenesis is dependent on regular expression of Zfp637.
(A) ICC showing that Zfp637 is highly expressed and localizes in the nucleus of GC-1 spg cells. (B) IHC, (C) qRT-PCR and (D) western blot analysis were performed to evaluate the expression of Zfp637 in mouse testis tissue. (E) Effects of OE-Zfp637 in GC-1 spg cells, Zfp637 expression knockdown by siRNA-Zfp63 and the expression of TH2B detected by qRT-PCR. (F) ICC was used to measure the expression of the specific haploid cell marker, protamine 1.