Figure 2 | Scientific Reports

Figure 2

From: IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway

Figure 2

Zfp637 regulates the differentiation of spermatogonia by inhibiting the expression of SOX2.

(A) ICC showing that Zfp637 and SOX2 are both expressed in GC-1 spg cells and localize in the nucleus. (B) Immunoprecipitation showing that Zfp637 and SOX2 protein do not physically interact with each other. (C) There is one Zfp637 binding site in the SOX2 promoter. (D) EMSA showing the formation of Zfp637 complexes with a probe derived from the SOX2 promoter. (E) Western blot showing that the expression of SOX2 is inversely correlated with Zfp637 expression. (F) Expression of SOX2 in mouse testicles by IHC. (G) Prevention of spermatogonial cell differentiation by overexpression of SOX2.

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