Figure 3

IL-6 can directly disrupt spermatogenesis by inhibiting the expression of Zfp637 in obese individuals.
The expressions of protamine 1 (A), TH2B (B) and Zfp637 (C) were measured by ICC, qRT-PCR and western blot, respectively, after a 48-h treatment with Leptin. (D) ELISA demonstrating elevated IL-6 levels in obese mice. (E) The expression levels of protamine 1, (F) TH2B and (G) Zfp637 measured by ICC-IF, qRT-PCR and western blot, respectively, after a 24-h treatment with IL-6.