Figure 5 | Scientific Reports

Figure 5

From: IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway

Figure 5

IL-6 reduces the expression of Zfp637 via the SOCS3/pSTAT3 signaling pathway.

(A) Activity of the SOCS3/STAT3 signaling pathway after exposure of GC-1 spg cells to IL-6 for 24 hours. * and #p < 0.01 compared with the IL-6 = 0 ng/ml group. ** and ##p < 0.0001 compared with the IL-6 = 0 ng/ml group. (B) Expression of STAT3, pSTAT3 and Zfp637 in GC-1 spg cells after OE-SOCS3. *p < 0.001. #p < 0.001. (C) IHC staining to measure the expression of SOCS3 in normal and obese mouse testes. (D) Cells treated with AG490 (10 nM, 2 hours) prior to exposure to IL-6. 1, 2, 3 and 4, p < 0.001 compared with the blank group. 1*, 2*, 3* and 4* p < 0.001 compared with the blank group. (E) Overexpression of Zfp637 did not influence the expression of STAT3, pSTAT3 or SOCS3.

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