Table 1 Summary of DNA extraction kit characteristics.
Extraction Method | Manufacturer | Principle | Costs per sample * [€] | Completion time * (hands-on-time) | Cell count | Yield [μg] (SD) | Purity [A260/280] (SD) |
|---|---|---|---|---|---|---|---|
Genomic-tip 20/G | Qiagen | anion-exchange column (gravity) | 8.1 | 8 h (45 min) | 4 × 109 | 9.8 (3.5) | 1.77 (0.06) |
MagAttract HMW DNA Kit | Qiagen | DNA-binding magnetic beads, silica-based | 4.4 | 2 h 40 min (1 h) | 2 × 109 | 10.3 (6.6) | 1.83 (0.05) |
MasterPure DNA Purification Kit | Epicentre | salting-out | 1.1 | 2 h 10 min (35 min) | 0.4 × 109 | 3.3 (1.0) | 1.82 (0.03) |
Wizard Genomic DNA Purification Kit | Promega | salting-out | 2.0 | 3 h (35 min) | 4 × 109 | 18.1 (7.5) | 1.58 (0.01) |
DNeasy Blood & Tissue Kit | Qiagen | silica-membrane column (spin) | 3.2 | 3 h (45 min) | 2 × 109 | 10.9 (1.3) | 1.72 (0.05) |
Plasmid Mini Kit | Qiagen | alkaline lysis, anion-exchange column (gravity) | 5.2 | 3 h 50 min (40 min) | 18 × 109 | 0.50 (0.2) | 1.67 (0.03) |
