Figure 1
From: IGF-1 protects tubular epithelial cells during injury via activation of ERK/MAPK signaling pathway
IGF-1 administration significantly ameliorates UUO-induced kidney injury.
(A) Experimental design: We performed UUO on mice, while some of which received daily i.p injection of IGF-1 (UUO+IGF-1), while the other received daily i.p. injection of saline as a control (UUO). Another group of mice that received sham operation were also daily i.p. injected with saline as another control (Sham). At 3 days after UUO, we digested the kidney from some of the mice for sorting of renal epithelial cells by fluorescence-activated cell sorting (FACS). These purified renal epithelial cells were further analyzed. At 14 days after UUO, the rest of the mice were sacrificed for histology analysis to confirm the model. (B,C) Masson trichrome staining was done to analyze and score for the degree of renal fibrosis regions in these mice, shown by representative images (B) and by quantification (C). N = 10. *p < 0.05.
