Figure 1
Multiple electrode array (MEA) recording of light response from TKO retina explants.
(A) Image showing micro ERG type responses recorded from the TKO retina following stimulation with 500 nm light (500 ms, 15.1 log photons/cm2/s). Panels show the responses recorded from all recording electrodes of a single MEA chamber. (B) An example of a micro ERG response from a single electrode showing the principle components of the ERG response (500 nm light, 500 ms, 15.1 log photons/cm2/s). Lower panels show the effect of 100 μm L-AP4 at reducing the b-wave component, leading to the isolation of the a-wave component (middle), which is rapidly bleached (right). (C) Recording from a single electrode showing the amplitude of micro ERG responses elicited by increasing intensities of 500 nm light (500 ms, 11.1 to 15.1 log photons/cm2/s). Lower panels show individual responses at higher resolution. (D) Graph showing the percentage of electrodes exhibiting micro ERG type light responses following 500 nm light pulses (500 ms, 15.1 log photons/cm2/s) and 360 nm light pulses (500 ms, 14.9 log photons/cm2/s). * Indicates p < 0.01. (E) Image showing micro ERG type responses recorded from the normal wildtype retina following stimulation with 500 nm light (500 ms, 15.1 log photons/cm2/s). (F) Image showing an example of micro ERG responses recorded from a single electrode in wildtype retina (500 nm light, 500 ms, 15.1 log photons/cm2/s). Note the increased amplitude of a-waves compared to TKO responses. (G) Image showing an example of micro ERG responses recorded from a single electrode in degenerate rd/rd cl retina lacking rods and cones (500 nm light, 500 ms, 15.1 log photons/cm2/s). Note the lack of micro ERG type responses in the rd/rd cl retina.
