Table 1 Primary and secondary antibodies.

From: Characterisation of light responses in the retina of mice lacking principle components of rod, cone and melanopsin phototransduction signalling pathways

Target

Antibody species

Antibody/Source

Dilution

Secondary antibody

Melanopsin

Rabbit polyclonal

UF006, Advanced Targeting Systems

1:2500

Donkey anti-rabbit Alexa 488

c-fos

Rabbit monoclonal

9F6, Cell signalling

1:200

Donkey anti-rabbit Alexa 568

c-fos

Sheep polyclonal

ab6167, Abcam

1:500

Donkey anti-sheep Alexa 568

β-gal

Chicken polyclonal

ab9361, Abcam

1:1000

Donkey anti-chicken Alexa 488

Brn3a

Goat polyclonal

sc-31985, Santa Cruz Biotech

1:1000

Donkey anti-goat Alexa 488

GS

Mouse monoclonal

MAB302, Millipore

1:1000

Donkey anti-mouse 488

Dab1

Rabbit polyclonal

LS-B9240, Lifespan Biosciences

1:1000

Donkey anti-rabbit Alexa 488

TH

Chicken polyclonal

ab76442, Abcam

1:1000

Donkey anti-chicken Alexa 488

GABA

Mouse monoclonal

GB-69, Sigma

1:2500

Donkey anti-mouse Alexa 488

GlyT-1

Goat polyclonal

AB1770, Millipore

1:1000

Donkey anti-goat Alexa 488/633

ChAT

Goat polyclonal

AB1449, Millipore

1:1000

Donkey anti-goat Alexa 488

Calbindin

Rabbit polyclonal

ab11426, Abcam

1:1000

Donkey anti-rabbit Alexa 488

CHX10

Sheep polyclonal

ab16141, Abcam

1:500

Donkey anti-sheep Alexa 488

  1. Details of primary antibodies used for immunohistochemistry. β-gal; beta-galactosidase, Brn3a; Brain-specific homeobox POU domain protein 3A, GS; Glutamine synthetase, Dab1; Disabled-1, TH; Tyrosine hydroxylase, GABA; Gamma-Aminobutyric acid, GlyT-1; Glycine Transporter-1, ChAT; Choline acetyltransferase, CHX10; Homeodomain transcription factor ChX10. Note that two different c-fos antibodies raised in different species were employed to allow double labelling with different combinations of primary antibodies.
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