Figure 3: HLA-DR4 (class II), but not HLA-A2 (class I), expression in NRG mice is required for development of human CD4 T and CD8 T cells able to secrete cytokines.

Panel (a) splenic cells from DRAGA (n = 6), A2 (n = 3) and DRAG (n = 2) mice were stimulated for 36 h with PMA/ionomycin, and the cytokines secreted in cell culture supernatants were measured by Luminex. Non-stimulated cells secreted < 50 pg/ml. Data present cytokine levels over the background (non-stimulated). To address the function of human CD4 and CD8 T cells from DRAGA and A2 mice, CD4 T cell-depleted or CD8 T cell-depleted spleen cells, or positively-isolated splenic CD4 T and CD8 T cells were stimulated as above. Data represent values of mice analyzed individually. Bars indicate average cytokine levels. Panel (b) splenic cells from additional groups of DRAGA (n = 6) and A2 (n = 4) mice were stimulated in vitro with anti-human CD3/CD28 coated beads and the levels of human IL-2, IFNγ, and TNFα secreted in cell culture supernantants were measured by luminex. Data represent values of mice analyzed individually. Bars indicate average cytokine levels.