Figure 1: UPLC analysis of immunoglobulin G (IgG) glycosylation.
From: Glycosylation of plasma IgG in colorectal cancer prognosis
Each IgG contains one conserved N-glycosylation site on Asn197 of its heavy chain. Different glycans can be attached to this site and the process seems to be highly regulated. UPLC analysis can reveal composition of the glycome attached to a population of IgG molecules by separating total IgG N-glycome into 24 chromatographic glycan peaks (GP1–GP24), mostly corresponding to individual glycan structures.
