Figure 2 | Scientific Reports

Figure 2

From: Antiproliferation of berberine is mediated by epigenetic modification of constitutive androstane receptor (CAR) metabolic pathway in hepatoma cells

Figure 2

Berberine induced apoptosis, cell cycle arrest and ROS production in absence or presence of mCAR in HepG2 cells.

(a–d): Berberine (BBR, 1, 5, 25 μM) induced apoptosis including early apoptosis (lower right quadrant) and necrosis (upper right quadrant) in HepG2 and mCAR-HepG2 cells for 24 h (a,b) and 48 h (c,d) by flow cytometry. (e–j): Effect of berberine (BBR, 1, 5, 25, 50 μM) on cell cycle distribution of HepG2 and mCAR-HepG2 cells for 24 h (e–g) and 48 h (h–j). (k–m): Berberine (BBR, 1, 5, 25, 50 μM) induced reactive oxygen species (ROS) production in HepG2 cells (k,m) or mCAR-HepG2 cells (l,m) for 24 h. (See also Supplementary Figure Legends).

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