Figure 4: Determination of the IC50 and IC90 of F-Asn to a fraB mutant. | Scientific Reports

Figure 4: Determination of the IC50 and IC90 of F-Asn to a fraB mutant.

From: A metabolic intermediate of the fructose-asparagine utilization pathway inhibits growth of a Salmonella fraB mutant

Figure 4

Wild-type (14028, closed circles), ΔfraB::kan (HMB206, open squares) or Δfra island (HMB205, closed triangles) were grown in M9 minimal medium containing 5 mM glucose and varying concentrations of F-Asn. The data points represent the optical density obtained after 15 h of growth at 37 °C (three biological replicates with three technical replicates in each (nine total replicates); error bars represent standard deviation. IC50 and IC90 were calculated and plotted ± the 95% confidence intervals. The IC50 is 19 μM (9 to 40 μM) and the IC90 is 174 μM (82 to 368 μM).

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