Figure 4
Development of electrical coupling between SST interneurons in the ACC.
Panel (A) shows the electrical coupling at different ages: P12, P21 and P45. Cells were held at approximately −70 mV with current clamp methods. Voltage hyperpolarization (up to approximately −50 mV) was induced by current injection. Panel (B) illustrates the age-dependent electrical coupling that was observed until P21–30 (42%). This coupling decreased at ≥P31 (~31%) to a value similar to that at P18–20 (~35%). The table in the panel (B) indicates the inter-somatic distance of cells (unit: μm). The upper row illustrates distance between coupled cells and lower row illustrates distance between all paired recorded cells. There is no significant difference between each group. The number of pairs of cells for each age group is indicated at the bottom of the bar. The cells are from a total of 27 mice. Panels (C,D) show no developmental change in conductance (GJ) or the coupling coefficient (CC). Two voltage-clamp examples used for measuring GJ are illustrated in panel (C). The detected values of I2 current were ~3 pA for P15 (left) and ~7 pA for P28 (right). One-way ANOVAs with Tukey’s post-hoc tests were used.
