Figure 8
Low concentrations of Resveratrol activates ERK1/2 and p38 signalling molecules in NPCs.
(a) Activation of the ERK1/2, p38 and JNK1/2, p-CREB, Bcl-2 and activated caspase-3 on exposure to different concentration of Resveratrol for 1 h. (b) Quantification was done in Gel Documentation System (Alpha Innotech, USA) with the help of AlphaEaseTM FC Stand-Alone V.4.0 software and expressed in fold change. (c) Activation of pTrKA, p75NTR, SIRT 1 on exposure to different concentrations of Resveratrol for 1 h. (d) Quantification was done in Gel Documentation System (Alpha Innotech, USA) with the help of AlphaEaseTM FC Stand-Alone V.4.0 software and expressed in fold change. (e) Activation of downstream genes of p-CREB viz. PSA-NCAM, Synaptophysin and NMDA R2 on exposure of Resveratrol for 1 h. (f) Quantification was done in Gel Documentation System (Alpha Innotech, USA) with the help of AlphaEaseTM FC Stand-Alone V.4.0 software and expressed in fold change. (g) Activation of the ERK1/2, p38 and JNK1/2 signalling molecules phosphorylation on exposure of 10 μM of Resveratrol for different time periods (1–6 h). (h) Activation of p-CREB, PSA-NCAM, Synaptophysin and NMDA R2 proteins on exposure of 10 μM Resveratrol for different time periods (1-6 h)β-actin was used as an internal control to normalize the data. *p < 0.05; **p < 0.01; ***p < 0.001.
