Figure 5
Watermelon plants were inoculated with Fon 1, ΔFon1SIX6, ΔFon1SIX6 + SIX6, Fon 2 and Fon 2 + SIX6 and the development of disease symptoms was assessed.
Seedlings (cv. Calhoun Gray, resistance to Fon 1) at the first true leaf stage were inoculated with a fungal spore suspension and disease symptoms were scored after 15 days. (a) The ΔFonSIX6 mutants were generated using Agrobacterium-mediated targeted disruption of the SIX6 gene. (b) Representative plants are shown 15 days post-inoculation. (c) Quantification of disease assays by weight. The outcomes of the disease assays depicted in (b) were quantified based on the average plant weight from each inoculation. Error bars indicate standard deviation and letters indicate values that are significantly different from each other (P < 0.01, all pairs Student’s t-test). All of the assays were repeated at least three times.
