Figure 2: Conidiation in the repression of FgHSP90 mutants. | Scientific Reports

Figure 2: Conidiation in the repression of FgHSP90 mutants.

From: Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum

Figure 2

(a) Conidial production. The number of conidia was counted after 5 days of incubation in CMC in the absence or presence of β-est. The values were generated based on three biological replicates. (b) Conidial morphology. Conidia were induced on YMA in the absence or presence of β-est and subsequently observed by DIC microscopy. Scale bar = 20 μm. (c) Percentage of conidial septa. One hundred conidia induced on YMA were assessed for each strain with three biological replicates. (d) Relative transcript levels of genes related to conidiation. Total RNA was extracted from the wild-type and HK226 strains cultured in CM for 48 h and then subjected to asexual induction in CMC for 6 h. The relative transcript levels of each gene in the wild-type strain were arbitrarily set to 1. (e) Morphology of conidiophores in F. graminearum strains. Images were captured 1 to 3 days after conidial induction on CMC in the absence or presence of β-est. Arrows and arrowheads indicate phialides and conidia directly produced from hyphae, respectively. Scale bar = 10 μm. (f) Cellular localisation of AbaA-Gfp in the strain with wild-type allele of FgHSP90 (left) and in the FgHSP90-repressed strain (right). Scale bar = 10 μm.

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