Figure 3: Virulence and sexual development.
(a) Virulence on wheat heads. The centre spikelet of each wheat head was injected with 10 μl of a conidial suspension. Images were captured 21 days after inoculation. (b) Micrographs of manually generated sections after infection of wheat. Wheat spikelets were inoculated with conidial suspensions from strains expressing Gfp in the cytoplasm (HK12 and HK302). Infected wheat heads were longitudinally dissected 6 days after inoculation and examined under a fluorescence microscope. Gfp fluorescence represents hyphae spreading from the inoculation points. Arrowheads mark the inoculated spikelets. Reflected, reflected light. (c) Sexual development. A five-day-old culture in carrot agar was mock-fertilised to induce sexual production and incubated for an additional 7 days. The upper and below panels show photographs of self-fertility on a whole carrot agar plate and photographs captured using a dissecting microscope, respectively. Scale bar = 500 μm.
