Figure 4: Localisation of FgHsp90 in F. graminearum.

Representative images showing co-localisation of FgHsp90 fused to Gfp with histone H1 fused to Rfp. (a) FgHsp90 translocated to nuclei from the cytosol after heat shock treatment at 37 °C for 1 h. Scale bar = 10 μm. (b) Localisation of FgHsp90 during conidiogenesis in CMC under optimal conditions (left) and heat shock treatment (37 °C for 1 h) (right). Scale bar = 10 μm. (c) A strain containing FgHSP90-GFP and hH1-RFP was grown on YMA (conidia), CM (germination and mycelia), or carrot agar medium (ascospores) for microscopic observation. DIC, differential interference contrast. Scale bar = 10 μm.