Figure 2

Identification of Netropsin.
(a) HPLC spectrum of Compound 1 from AN130070 metabolite (left) and netropsin (right). Inner panel presented UV absorption of each peaks originated from subfraction of AN130070. RB625B12;Varian C18 column; 5~30% MeCN for 20 min, ~60% MeCN for 30 min, ~100% MeCN for 5 min, wash for 10 min, flow rate 1 mL/min, detection by absorption at 297 nm (b) Mass spectrum (positive ESI mode) of Compound 1 (Hao et al. 2013).