Figure 2: IRS1KO β-cells shows decreased nuclear accumulations of sXBP-1. | Scientific Reports

Figure 2: IRS1KO β-cells shows decreased nuclear accumulations of sXBP-1.

From: IRS1 deficiency protects β-cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translation

Figure 2

(a–c) Control, IRS1KO, and IRS2KO β-cells incubated with low glucose (2.5 mM), high glucose (25 mM), tunicamycin (2 μg/ml), or thapsigargin (100 nM) for 4 h after overnight starvation. (a) Immunoblot of sXBP-1 and lamin B1 (loading control) in nuclear fractions. Data are means ± SEM, n = 3. *P < 0.05. (b) Immunoblot of p-IREα, IRE1α and tubulin (loading control) using cytosolic fractions. Data are means ± SEM, n = 3. *P < 0.05. (c) Immunoblot of p-PERK, PERK, and β-actin using cytosolic fractions. Data are means ± SEM, n = 3. *P < 0.05.

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