Figure 6: Quantification of gene expression levels of 18 selected soybean CIPK genes under drought stress using qRT-PCR. | Scientific Reports

Figure 6: Quantification of gene expression levels of 18 selected soybean CIPK genes under drought stress using qRT-PCR.

From: Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean

Figure 6

Fifteen-day-old plants were subjected to drought stress by withholding water for 0 (control), 4, 8 and 12 days. Leaf, stem and root samples were collected at these four time points for RNA extraction and qRT-PCR quantification of the expression levels of 14 intron-poor genes (top panels) and 4 intron-rich genes (bottom panels). Ribosomal protein s20e gene (Glyma.03G142300) was used as an internal control to normalize gene expression levels. The fold-change values represent changes of mRNA levels in drought stressed samples relative to non-stressed control samples. Data are average of three independent biological samples ± SE. Mean values significantly different from the control plants were determined by t tests (P < 0.05) and are indicated by an asterisk.

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