Figure 9
CUR interferes with the oligomerization of Hla induced by deoxycholate.
(a) The influence of CUR on the hemolysis induced by Hla and its two mutants (*indicates P < 0.05 and **indicates P < 0.01; two tailed Student’s t-test). (b) CUR reduces the oligomer of Hla. The oligomerization of Hla and its two mutants was determined by western blot analysis after treated with 5 mM deoxycholate in the presence or absence of CUR. Lane 1, WT-Hla without 5 mM deoxycholate; lane 2–4, WT-Hla, Q89A-Hla and K163A-Hla treated with 5 mM deoxycholate, respectively; lane 5–7, WT-Hla, Q89A-Hla and K163A-Hla treated with 5 mM deoxycholate in the presence of 16 μg/ml of CUR, respectively.
