Figure 2: Phylogenetic analysis of the VirB4-like proteins of the plasmid dataset.

The analysis includes 13 proteins belonging to the CagE-TrbE-VirB pfam ID in Table S3 and representatives of the eight mating pair formation systems defined in Guglielmini et al.³⁵. The MPF_T cluster is shadowed in grey. VirB4-like proteins of the plasmid dataset are boxed.