Figure 4
miR-203 suppresses cell migration and invasion in vitro and in vivo.
(A) Representative pictures show the migrated SW620 and LOVO cells (left) and quantification of the number of migrated tumor cells (right). (B) Representative pictures of the matrigel invasion assay were obtained for the SW620 and LOVO cells (left) and the number of invasive tumor cells was quantified (right). (C) Western blot analysis of the relative protein levels of MTA1, RAC1, MMP-9, vimentin, β-catenin and E-cadherin in the miR-203 and control groups for both the SW620 and LOVO cell lines. (D) Representative hematoxylin and eosin staining of the lungs isolated from mice injected with the LOVO-vector or LOVO-miR-203 cells. The number of metastatic nodules in the lung was counted in 8 randomly selected high-power fields under a microscope. **p < 0.01.
