Figure 5
Antibacterial gene expression in monocytes/macrophages stimulated with IFNγ and infected with F. noatunensis.
(A) Monocytes/macrophages were treated with recombinant IFNγ or recombinant control (rCtr; back-ground proteins isolated from E. coli containing an empty vector) and analysed for gene expression of g-type lysozymes (LygF1a + b and LygF1c + d) and antibacterial genes (cathelicidin and hepcidin). (B,C) Monocytes/macrophages were either infected with F. noatunensis (F.n.), pre-treated by recombinant rCtr or IFNγ prior to infection or left un-treated (Ctr) and subjected to gene expression of analyses (B) g-type lysozymes (LygF1a + b and LygF1c + d) and (C) and antibacterial genes (cathelicidin and hepcidin). Expression of target genes were normalised to eF1a expression and calibrated to the Ctr cells at 6 h. Results are shown as relative quantification values obtained from four fish with mean quantity and calculated SEM. The asterisks above columns indicate significant differences (p < 0.05) compared to the F. noatunensis infected cells at the same sampling point.
