Figure 2 | Scientific Reports

Figure 2

From: EMMPRIN Down-regulating miR-106a/b Modifies Breast Cancer Stem-like Cell Properties via Interaction with Fibroblasts Through STAT3 and HIF-1α

Figure 2

Recombinant EMMPRIN increases breast cancer cells to show stem-like cell properties.

EMMPRIN increased the mammosphere formation of breast cancer cells. SUM102 (A) and BT474 (B) breast cancer cells were treated with hrEMMPRIN and the cells were cultured in stem cell specific medium for 10 days and then numbers of sphere were counted. (C) hrEMMPRIN increased CD44 expression and decreased CD24 expression in breast cancer cells. BT474 breast cancer cells were treated with human recombinant EMMPRIN and then the cells were fixed for immunofluoresence assay. (D) hrEMMPRIN increased the percentage of CD44+CD24− breast cancer cells. BT474 breast cancer cells were treated with human recombinant EMMPRIN and then the cells were collected for flow cytometry analysis. (E) Data analysis from D. The data presented were shown as means ± s.d. collected from three independent experiments. *p < 0.05, **p < 0.01.

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