Figure 2

Physical mapping of eight of the long satDNAs found in Locusta migratoria, showing the three patterns of chromosome distribution observed: non-clustered (a), clustered (d–h) and mixed (b–c). All cells showed here (except that in (g)) are mitotic metaphase haploid cells from haplo-diploid embryos obtained in our laboratory. The cell in (g) is at meiotic metaphase I and was obtained from an adult male. Each cell is shown in red color for satDNA FISH (upper panel) and merged with DAPI (lower panel). In (f,g), double FISH was performed to distinguish whether the sat-carrying chromosome was M6 (harboring rDNA shown in green) or any other medium-sized chromosome. Note in (h) the presence of LmiSat01–193 in the pericentromeric regions of all chromosomes.