Figure 3 | Scientific Reports

Figure 3

From: The chimeric ubiquitin ligase SH2-U-box inhibits the growth of imatinib-sensitive and resistant CML by targeting the native and T315I-mutant BCR-ABL

Figure 3

SH2-U-box inhibits the growth of K562 and K562R cells and exerts additive effect with imatinib in K562 cells.

(A,B) K562 and K562R cells stably expressing eGFP, SH2 and SH2-U-box were incubated with or without 0.5 μM imatinib for 5 days. Cell growth were measured by CCK-8 assay (n = 6). (C–F) K562 and K562R cells were transiently electropored with CMV, SH2- or SH2-U-box-encoding plasmids and 24 hours later, incubated with or without 0.5 μM imatinib for 24 hours. Cell cycle was examined by flow cytometric analysis (n = 3) (C,D) and cell apoptosis was determined by PI-Annexin V staining followed by flow cytometric analysis (n = 3)(E,F). Each experiment was repeated at least three times independently and the representative results are the mean ± S.D. from six-replicates (A,B) or triplicate (C–F). *p < 0.05, ***p < 0.001, Student’s t test.

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