Figure 3
HSP60 knockdown disrupts complex I integrity leading to ROS overproduction.
(a) Graphical representation of TMT ratios for subunits of respiratory complex I in HSP60-KN-U87 cells compared to the control cells. (b) Graphical representation of TMT ratios for newly synthesized subunits of respiratory complex I in HSP60-KN-U87 cells compared to the control cells. (c) Graphical representation of ROS levels of HSP60-KN-U87 cells compared to the control cells. (d) Graphical representation of ROS levels of U87 cells treated with 100 nM rotenone for 1 h. (e) Growth of U87 cells was suppressed when treated with different concentrations of rotenone for 24 h. (f) Growth curve of U87 cells treated with different concentrations of rotenone. Data were analyzed using student’s t test. *p < 0.05, **p < 0.01 and ***p < 0.001. *p < 0.05 is considered statistically significant. Error bars represent ± SEM.
