Figure 5

Lipid synthesis was decreased in HSP60-KN-U87 cells compared to the control cells.
(a) Expression levels of proteins in lipid metabolism were down-regulated in HSP60-KN-U87 cells compared to the control cells. (b) Lipidomics analysis showed that five classes of phospholipids were decreased in HSP60-KN-U87 cells compared to the control cells. Data were analyzed using student’s t test. *p < 0.05, **p < 0.01 and ***p < 0.001. *p < 0.05 is considered statistically significant. Error bars represent ± SEM. (c,d) Nile red staining of the control cells with or without bright-field alignment. (e,f) Nile red staining of HSP60-KN cells with or without bright-field alignment.