Figure 6
HSP60-silencing promoted cells to undergo the EMT process.
(a) Graphical representation of TMT ratios for newly synthesized proteins in Wnt/β-catenin pathway that were up-regulated in HSP60-KN-U87 cells. (b) Western blotting images of β-catenin, vimentin, N-cadherin in the control cells and HSP60-KN cells. (c) Cell invasion assay showed that HSP60-KN cells displayed a higher invasiveness than control cells did. Data were analyzed using student’s t test. *p < 0.05, **p < 0.01 and ***p < 0.001. Error bars represent ± SEM. (d) Western blotting images of AMPK, vimentin, N-cadherin of untreated- and rotenone-treated U87 cells for 24 h.
