Figure 4: Knockdown of CHIP enhances DOX-induced cell apoptosis, pro-inflammatory cytokine expression and oxidative stress in neonatal rat cardiomyocytes. | Scientific Reports

Figure 4: Knockdown of CHIP enhances DOX-induced cell apoptosis, pro-inflammatory cytokine expression and oxidative stress in neonatal rat cardiomyocytes.

From: Protection against doxorubicin-induced myocardial dysfunction in mice by cardiac-specific expression of carboxyl terminus of hsp70-interacting protein

Figure 4

(a) Cardiomyocytes were infected with adenovirus siRNA-control or siRNA-CHIP and then treated with different doses of 0.5, 1 or 5 μM DOX for 24 hours. Cell viability was measured by trypan blue exclusion assay (top, scale bar = 100 μm). Quantitative analysis of viable cells (bottom). (b) Apoptosis was detected by TUNEL assay 24 hours after DOX (0.5 μM) treatment (left, scale bar = 50 μm). Quantitative analysis of TUNEL-positive cells (right). (c) qPCR analysis of Bax and Bcl-2 mRNA expression was performed, and Bax/Bcl-2 ratio was determined for each group. (d) qPCR analysis of proinflammtory cytokines IL-1β, IL-6, TNF-α mRNA expression was performed. (e,f) MDA levels and GPx activities were measured in cardiomyocytes homogenates from siRNA-control and siRNA-CHIP infected groups. *P < 0.05 versus siRNA-control + saline. #P < 0.05 versus siRNA-control + DOX.

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