Figure 5
From: Involvement of Cl−/HCO3− exchanger SLC26A3 and SLC26A6 in preimplantation embryo cleavage
Effect of SLC26A3 and/or SLC26A6 knockdown on embryo cleavage and HCO3−-dependent signalling cascade.
(A,B) Quantitative real-time PCR result showing the expression of SLC26A3 (A) and SLC26A6 (B) mRNA in embryos after control siRNA (siRNA NC), SLC26A3 siRNA, SLC26A6 siRNA or both SLC26A3 and SLC26A6 siRNA injection at the 2-cell stage. *** indicates P < 0.001 (by one-way ANOVA, n = 4). (C) Four-cell embryo formation after 12 hours of embryo culture following siRNA NC, SLC26A3 siRNA, SLC26A6 siRNA or both SLC26A3 and SLC26A6 siRNA injection at 2-cell stage (siRNA NC: 32/45 embryos; SLC26A3 siRNA: 11/44 embryos; SLC26A6 siRNA: 21/40 embryos; SLC26A3 + A6 siRNA: 5/42 embryos). Scale bar: 100 μm. (D) Summary of the results from C. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001 (by one-way ANOVA, n = 4). (E–G) Quantitative real-time PCR showing the expression of miRNA-125b (E), p53 (F) and p21 (G) after siRNA NC, SLC26A6 siRNA, SLC26A3 siRNA or both SLC26A3 and SLC26A6 siRNA injection. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001 (by one-way ANOVA, ~100 embryos in each experiment, n = 4).
