Figure 4: Dependence of chemical shift and cross peak volumes on the xenon concentration. | Scientific Reports

Figure 4: Dependence of chemical shift and cross peak volumes on the xenon concentration.

From: Structural transitions in full-length human prion protein detected by xenon as probe and spin labeling of the N-terminal domain

Figure 4

The sample contained 0.25 mM of 15N-enriched huPrP(23–230) in 5 mM acetate buffer, 0.1 mM DSS, pH 4.5 in 90% 1H2O/10% 2H2O. Temperature 293 K. For residues typical for the various cavities (left) the relative intensity changes −ΔV/V0 and (right) the combined chemical shift changes Δδcomb are plotted as a function of the free xenon concentration c. The curves shown were calculated with the parameters of Table 3, only the chemical shifts in the different states are free fit parameters.

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