Figure 6: Paramagnetic effects in spin labelled full-length prion protein. | Scientific Reports

Figure 6: Paramagnetic effects in spin labelled full-length prion protein.

From: Structural transitions in full-length human prion protein detected by xenon as probe and spin labeling of the N-terminal domain

Figure 6

The relative change −ΔVmax/V0 = (V0 − Vmax)/V0 of cross peak volumes as a function of the sequence position j in [1H, 15N]-HSQC spectra of 15N-enriched huPrP(23–231) variants G93C (a) and T107C (b) labeled with methanesulfonothioate and maleimide based nitroxyl spin label, respectively. Solid and broken lines represent standard deviations σ0 and 2σ0. Proline residues are marked with P. Additionally invisible and unassignable residues in the spectra are marked with X. V0 corresponds to the cross peak volume after reduction of the spin label and Vmax the cross peak volume after labeling (see Materials and Methods).

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