Figure 7: Mapping of paramagnetic effects on spin labeled full-length prion protein.

Paramagnetic effects of MTSL and maleimide-linked nitroxyl spin label on huPrP variants G93C (a) and T107C (b), respectively. Very strong paramagnetic effects > 2 σ0 are colored in orange, strong effects > σ0 in red, small and negative effects < σ0 are colored in blue and unassigned residues in grey. The labeling position is displayed as spheres and the alanine-rich region in sticks. Mapping of the paramagnetic effects and in silico mutagenesis was done with Pymol (Schrödinger LLC) and the first structure of PDB entry 2KUN.