Figure 1: PIAS4 up-regulation and SIRT1 down-regulation accompany steatosis-associated liver fibrosis in mice.

Male db/db mice were fed on the MCD diet or a control diet (chow) for 4 weeks. (A,B) Expression of SIRT1 and PIAS4 was examined by qPCR (A) and Western blotting (B). (C) Binding of PIAS proteins to the SIRT1 promoter was evaluated by ChIP.