Figure 2: PIAS4 mediates transcriptional repression of SIRT1 by high glucose in hepatic stellate cells. | Scientific Reports

Figure 2: PIAS4 mediates transcriptional repression of SIRT1 by high glucose in hepatic stellate cells.

From: Transcriptional repression of SIRT1 by protein inhibitor of activated STAT 4 (PIAS4) in hepatic stellate cells contributes to liver fibrosis

Figure 2

(A–C) Primary mouse hepatic stellate cells were treated with glucose (35 mM) or low glucose (5.5 mM). mRNA and protein levels were measured by qPCR (A) and Western (B). (C) PIAS binding to the SIRT1 promoter was examined by ChIP. (D) A SIRT1 promoter-luciferase construct was transfected into HSC-T6 cells along with indicated PIAS expression constructs followed by treatment with high glucose for 24 hours. Luciferase activities were normalized to protein concentration and GFP fluorescence for transfection efficiency and expressed as relative luciferase activity compared to the control group. (E,F) Primary hepatic stellate cells were transfected with indicated siRNAs followed by treatment with glucose. mRNA (E) and protein (F) levels of SIRT1 were measured by qPCR and Western.

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