Figure 3: PIAS4 knockdown restores SIRT1 expression and alleviates liver fibrosis in mice. | Scientific Reports

Figure 3: PIAS4 knockdown restores SIRT1 expression and alleviates liver fibrosis in mice.

From: Transcriptional repression of SIRT1 by protein inhibitor of activated STAT 4 (PIAS4) in hepatic stellate cells contributes to liver fibrosis

Figure 3

Male db/db mice were fed with indicated diets for 4 weeks. Silencing of PIAS4 was mediated by lentivirus as described under Methods. (A,B) Expression of PIAS4 and SIRT1 was measured by qPCR (A) and Western (B). (C,D) Paraffin embedded liver sections were stained with picrosirius red (C) or Masson’s trichrome (D). (E,F) Expression of fibrogenic proteins was measured by qPCR (E) and Western (F).

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