Figure 4: PIAS4 knockdown suppresses SMAD3 acetylation and target binding in mice. | Scientific Reports

Figure 4: PIAS4 knockdown suppresses SMAD3 acetylation and target binding in mice.

From: Transcriptional repression of SIRT1 by protein inhibitor of activated STAT 4 (PIAS4) in hepatic stellate cells contributes to liver fibrosis

Figure 4

Male db/db mice were fed with indicated diets for 4 weeks. Silencing of PIAS4 was mediated by lentivirus as described under Methods. (A,B) Binding of SIRT1 (A) or SMAD3 (B) to fibrogenic gene promoters was examined by ChIP. (C) Liver homogenates were immunoprecipitated with anti-SMAD3 and the precipitated immune complex (eluate) was separated by SDS-PAGE gel electrophoresis. Western blotting was performed with indicated antibodies.

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