Figure 5
From: A new autoinhibited kinase conformation reveals a salt-bridge switch in kinase activation
Effects of free Mg2+ on the activation of EphA2, PKA and p38MAPK mutants.
Each experiment was performed in triplicate (the error bar represents standard deviation). (a) Reaction velocity of the EphA2 mutants Tyr772Phe (black) and Arg762Ala/Tyr772Phe (red) are measured at increasing Mg2+ concentration in the range of 0–10 mM. (b) Reaction velocity of the PKA mutants Thr197Ala (black) and Lys189Ala/Thr197Ala (red) are measured in a similar manner to EphA2. (c) Reaction velocity of the p38MAPK mutants Thr180Ala (black) and Arg173Ala/Thr180Ala (red) are measured at Mg2+ concentrations in the range of 0–2 mM.
